Journal:

Article Title: Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

doi: 10.1128/JCM.42.12.5549-5557.2004

Figure Lengend Snippet: Reference bacterial isolates used, and their nucleotide sequence accession numbers, and amplicon sizes in the lpxA multiplex PCR assay

Article Snippet: Reference bacterial isolates used, and their nucleotide sequence accession numbers, and amplicon sizes in the lpxA multiplex PCR assay Campylobacter concisus ATCC 33237, ATCC 43643, ATCC 51561, and ATCC 51562; Campylobacter curvus ATCC 35224, ATCC 33237, and RM3269; Campylobacter fetus subsp. fetus NCTC 10842, RM2086, RM2087, and RM2088; Campylobacter fetus subsp. venerealis NCTC 10354, RM3224.

Techniques: Sequencing, Amplification, Multiplex Assay

Journal:

Article Title: Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

doi: 10.1128/JCM.42.12.5549-5557.2004

Figure Lengend Snippet: Arrangement of the lpxA locus within the Campylobacter jejuni F38011 genome. The lpxA gene, flanked at the 5′ end by fabZ and at the 3′ end by clpX (direction of transcription shown by the arrows), is shown. The numbers indicate the nucleotide sequence obtained from the F38011-derived expression plasmid pCI1. Also indicated (solid arrowheads underneath the gene designations) are the relative positions of the PCR and sequencing primers lpxA0301F and lpxA0304R. The relative position of the species-specific primer positions are indicated as follows: Cc, C. coli; Cj, C. jejuni; Cl, C. lari; and Cu, C. upsaliensis, with LpxAKK2Rm representing the universal lpxA reverse primer.

Article Snippet: Reference bacterial isolates used, and their nucleotide sequence accession numbers, and amplicon sizes in the lpxA multiplex PCR assay Campylobacter concisus ATCC 33237, ATCC 43643, ATCC 51561, and ATCC 51562; Campylobacter curvus ATCC 35224, ATCC 33237, and RM3269; Campylobacter fetus subsp. fetus NCTC 10842, RM2086, RM2087, and RM2088; Campylobacter fetus subsp. venerealis NCTC 10354, RM3224.

Techniques: Sequencing, Derivative Assay, Expressing, Plasmid Preparation

Journal:

Article Title: Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

doi: 10.1128/JCM.42.12.5549-5557.2004

Figure Lengend Snippet: Phylogenetic analysis of the lpxA gene of 53 isolates of thermotolerant Campylobacter spp. The lpxA gene from isolates of C. coli, C. jejuni, C. lari, and C. upsaliensis was amplified from genomic DNA by PCR with primers lpxAF0301 and lpxAR0304. Amplicons were sequenced, and the primer regions were removed to facilitate the alignment. In total, 746 bp of C. jejuni, and C. lari nucleotide sequence, 754 bp of C. upsaliensis nucleotide sequence, and 757 bp of C. coli nucleotide sequence were aligned. The lpxA gene of Helicobacter hepaticus (ATCC 51499) was used to root the tree. The scale at the bottom of the figure is a measure of genetic identity. Numbers at the branches indicate the percent bootstrap support for each node. Note that the following isolates were not included in the figure for clarity; the lpxA sequences from these isolates were represented as follows. C. coli RM3232 also represents isolates RM3230 and RM3231 and M275 also represents isolates RM1051, RM1166, RM1505, RM1530, RM1531, RM1533, RM2225, and RM2228. C. jejuni RM3664 also represents isolates RM3665, RM3666, and RM3667; NCTC 11168 also represents ANR0493; RM3668 also represents RM3669 and RM3670; and F38011 also represents isolates RM1221, RM3672, and RM3673. C. lari RM2100 also represents RM2099, RM2808, RM2809, RM2817, RM2820, and RM2826 and RM1890 also represents RM2810 and RM2821. C. upsaliensis: RM1488 also represents RM2094.

Article Snippet: Reference bacterial isolates used, and their nucleotide sequence accession numbers, and amplicon sizes in the lpxA multiplex PCR assay Campylobacter concisus ATCC 33237, ATCC 43643, ATCC 51561, and ATCC 51562; Campylobacter curvus ATCC 35224, ATCC 33237, and RM3269; Campylobacter fetus subsp. fetus NCTC 10842, RM2086, RM2087, and RM2088; Campylobacter fetus subsp. venerealis NCTC 10354, RM3224.

Techniques: Amplification, Sequencing

Journal:

Article Title: Differentiation of Campylobacter coli , Campylobacter jejuni , Campylobacter lari , and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA

doi: 10.1128/JCM.42.12.5549-5557.2004

Figure Lengend Snippet: Multiplex PCR analysis of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. Species-specific PCR amplicons were resolved after electrophoresis through a 3% agarose gel. Lane 1, C. coli (391 bp); lane 2, C. jejuni (331 bp); lane 3, C. lari (233 bp); and lane 4, C. upsaliensis (206 bp). A DNA ladder (in base pairs) is shown on the left-hand edge of the gel.

Article Snippet: Reference bacterial isolates used, and their nucleotide sequence accession numbers, and amplicon sizes in the lpxA multiplex PCR assay Campylobacter concisus ATCC 33237, ATCC 43643, ATCC 51561, and ATCC 51562; Campylobacter curvus ATCC 35224, ATCC 33237, and RM3269; Campylobacter fetus subsp. fetus NCTC 10842, RM2086, RM2087, and RM2088; Campylobacter fetus subsp. venerealis NCTC 10354, RM3224.

Techniques: Multiplex Assay, Electrophoresis, Agarose Gel Electrophoresis

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Mucosa-associated bacteria isolated from child intestinal biopsies using the Ham's F-12 enrichment-filtration method.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Bacteria, Isolation, Infection

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Comparison of the percentage invasion and adherence into Caco-2 cells of eight Campylobacter concisus strains.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Comparison

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: C. concisus UNSW2 was observed as spiral curved-shaped bacteria with rounded ends and a single polar flagellum as shown in Panel A (bar = 3 µm). In Panel B (bar = 1.5 µm) C. concisus UNSW3 was observed to be curved-shaped bacteria with rounded ends and a single polar flagellum, while in Panels C (bar = 2 µm) and D (bar = 2.5 µm) C. concisus strains UNSW1 and UNSWCD were shown to be spiral curved-shaped bacterium with rounded ends and a single flagellum.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Bacteria

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Panel A shows an overview of uninfected Caco-2 monolayer. The Caco-2 cells expressed differentiating microvilli (Panel A1) and differentiated microvilli (Panel A2). C. concisus was shown to aggregate upon interaction with host cells as shown in Panel B (Panel B1, bar = 1.5 µm and Panel B2, bar = 2 µm). In Panel C, the polar flagellum of C. concisus is shown binding to the tips of host cell microvilli which mediated initial contact with host cells (as indicated by the arrows). Abnormalities in the epithelial host cell structure and microvilli were observed following infection with C. concisus (indicated by a ring in Panel D and arrows in Panel E). Panel F shows the flagellum of C. concisus appeared to wrap itself around the microvilli (as indicated by arrows). Following adherence, C. concisus induced a “membrane ruffling”-like effect on the host cell membrane (indicated by an asterisk in Panel F), and penetrated the host cell membrane from the non-flagellated end (indicated by an arrow in Panel G). C. concisus was observed invading the host cell (indicated by arrows in Panels G, H and I) resulting in irregular shaped membrane protrusions (indicated by asterisks in Panels G, H and I), leading to host cell damage (indicated by “#” in Panels G, H and I).

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Binding Assay, Infection, Membrane

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Panel A shows uninfected LS174T monolayers. LS174T cells expressing microvilli (indicated by a ring) and goblet cells (indicated by a arrow) are shown in Panel B. The mucus layer was found on the monolayer surface of LS174T cells as indicated by an “#” in Panel C. C. concisus appeared to be attracted to the mucus layer of host cells (indicated by “#” in Panel D) using their single polar flagellum (indicated by arrows in Panel E) and upon the interaction with host cells tended to aggregate (Panel F). Panel G shows the polar flagellum (as indicated by an arrow) of C. concisus binding to the tips of host cell microvilli (as indicated by a ring) and goblet cells (as indicated by an arrow in Panel H) which appeared to mediate initial contact with host cells. Following adherence, C. concisus induced a “membrane ruffling”-like effect on the host cell membrane (indicated by an asterisk in Panel I) and penetrated the host cell membrane from the non-flagellated end (indicated by an arrow in Panel I) resulting in cell damage (indicated by “#” in Panel I).

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Binding Assay, Membrane

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Lane 1: FN-1 marker, lane 2: UNSWCD, lane 3: UNSW2, lane 4: UNSW3, lane 5: UNSW1, lane 6: BAA-1457, lane 7: UNSWCS, lane 8: ATCC 51562, lane 9: ATCC 51651 and lane 10: negative control.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Marker, Negative Control

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Caco-2 cell proteins whose expression is upregulated in the presence of Campylobacter concisus UNSWCD.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Clinical Proteomics, Membrane, Binding Assay, RNA Binding Assay, Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: Caco-2 cell proteins whose expression is downregulated in the presence of Campylobacter concisus UNSWCD.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Expressing, Clinical Proteomics, Membrane, Binding Assay, RNA Binding Assay, Ubiquitin Proteomics

Journal: PLoS ONE

Article Title: The Pathogenic Potential of Campylobacter concisus Strains Associated with Chronic Intestinal Diseases

doi: 10.1371/journal.pone.0029045

Figure Lengend Snippet: (A) Non-invasive C. concisus strains adhere to the host cell and induce the production of IL-12. (B) Invasive C. concisus strains adhere to and invade the host cell inducing both IL-12 and IFN-γ, which in turn activate the immunoproteosome. The bacterial insult upregulates ubiquitinating and downregulates de-ubiquitinating enzymes which leads to the ubiquitination of NF-κB inhibitors. The immunoproteosome targets these inhibitors which activates NF-κB.

Article Snippet: Campylobacter concisus strains UNSWCD, UNSW1, UNSW2, UNSW3, ATCC 51561, ATCC 51562, UNSWCS and BAA-1457 were used in this study.

Techniques: Ubiquitin Proteomics

Journal: iScience

Article Title: Alterations in oligodendrocyte transcriptional networks reveal region-specific vulnerabilities to neurological disease

doi: 10.1016/j.isci.2023.106358

Figure Lengend Snippet:

Article Snippet: pcDNA1 Flag ATXN1[85Q] , Skinner et al. , Addgene plasmid #33237.

Techniques: Sample Prep, Plasmid Preparation, Software